Comparison of loop-mediated isothermal amplification and real-time PCR for detecting Bordetella pertussis.

Loop-mediated isothermal amplification (LAMP) is a rapid, sensitive and specific technique for detecting various infectious diseases. In the present study, we developed a LAMP assay based on insertion sequence IS481 of Bordetella pertussis (IS481-LAMP). We performed a LAMP assay of the PT promoter region of B. pertussis (PT-LAMP), and the results were compared with those obtained using a conventional real-time PCR based on IS481. Compared with the real-time PCR assay, the specificity of PT-LAMP and IS481-LAMP were 94.1% and 92.9%, and sensitivity were 76.2% and 87.5%, respectively. Therefore, real-time PCR is the most reliable method for detecting B. pertussis. However, PT-LAMP and IS481-LAMP may be alternative methods that can be used for the diagnosis of B. pertussis in clinical laboratories

 

Authors:Allahyar Torkaman MR1, Kamachi K2, Nikbin VS1, Lotfi MN1, Shahcheraghi F1.

Journal:J Med Microbiol. 2015

Link:http://www.ncbi.nlm.nih.gov/pubmed/25596118