The mouse intranasal challenge model for potency testing of whole-cell pertussis vaccines.

A mouse intracerebral challenge model is used for potency testing of whole-cell pertussis (wP) vaccines. We investigated the use of a mouse nasopharyngeal challenge model, which better reflects the clinical features of pertussis disease, to differentiate between efficacy of wP vaccines. Methods: Efficacy of three wP vaccines (Quinvaxem®, Easyfive® and Pentavac®) was tested in the nasopharyngeal challenge model. Mice were vaccinated at 4 and 7 weeks and challenged with Bordetella pertussis at 9 weeks. Vaccine efficacy was determined based on CFU in the lungs 5 days after challenge. Results: The mouse nasopharyngeal challenge model has the capacity to differentiate between the efficacy of whole cellpertussis vaccines. Conclusion: The mouse nasopharyngeal challenge model could be considered as a potency and release assay for wP vaccines. Whether this model directly correlates with clinical vaccine efficacy requires further investigations. Whether this model directly correlates with clinical vaccine efficacy requires further investigations. The mouse nasopharyngeal challenge model could be considered as a potency and release assay for wP vaccines.

 

Authors:Queenan AM1, Fernandez JShang WWiertsema Svan den Dobbelsteen GPPoolman J.

Journal:Expert Rev Vaccines. 2014

Link:http://www.ncbi.nlm.nih.gov/pubmed/25029905